MLS® Laser Therapy in bronchopulmonary dysplasia

An advanced model for studying lung inflammation

The study analyses the effect of near-infrared (NIR) PBMT in an ex vivo model of bronchopulmonary dysplasia (BPD), using ASA devices based on MLS^®(Multi-Wave Locked System) technology and lung tissue that preserves its architecture and biological complexity.
The primary endpoints considered are two key markers of inflammation:

IL-6

TNF-α

The objective is not to demonstrate the "generic" efficacy of PBMT, but to understand how specific energy delivery modes influence the biological response, specifically using MLS^®Laser Therapy.

Results: delivery mode matters

The data reveal several significant findings:

Protocols with lower power density and longer exposure times significantly reduce IL-6 and TNF-α
The effect is most evident at 2 hours,with attenuation at 6 hours
For the same total energy, biological responses differ significantly

In summary, a key concept emerges: it is not just the dose (energy density J/cm²) that determines the effect, but the way the energy is delivered.

Consistency with the biphasic model and ASA Research

These results fit consistently with the biphasic response model of PBMT, according to which:

stimuli that are too intense or too rapid can reduce biological efficacy
a more gradual administration of energy can promote the modulation of inflammatory pathways

The study also connects to an already established line of research:

previous proteomic evidence has suggested the involvement of regulatory proteins such as NLRP10
preliminary respiratory studies have indicated a possible anti-inflammatory effect in the lungs

This work adds a significant element: it provides direct evidence, in a controlled model, of the modulation of key inflammatory markers.

Limitations and interpretation

It is important to contextualise the results:

in vitro/ex vivo, non-clinical model
limited number of samples
analysis of a limited number of biomarkers

The study should therefore be considered exploratory and hypothesis-generating, but it represents a methodologically relevant step.

The evolution of photobiomodulation (PBMT) increasingly depends on a conscious and targeted management of parameters, supported by solid experimental evidence.