CELL GROWTH MODULATION OF HUMAN CELLS IRRADIATED IN VITRO
WITH LOW-LEVEL LASER THERAPY
MADDALENA MOGNATO, Ph.D., FRANCESCA SQUIZZATO
Dipartimento di Biologia, Università degli Studi di Padova
FRANCESCA FACCHIN,M.D., LUIGI CORTI, M.D.
Divisione di Radioterapia e Medicina Nucleare, Azienda Ospedaliera Università degli Studi di Padova LUCIO ZAGHETTO – Asa srl
Objective: The aim or this study was to investigate the effects or different wavelengths and doses or laser radi¬ation on in vitro cell proliferation. We evaluated the biological effects of low-level laser therapy (LLLT) on two human cancer cell lines: HeLa (epithelial adenocarcinoma) and TK6 (lymphoblast). Our attention was fo¬cused on the combination of the two laser emissions as it could have a synergic effect greater than the single emission applied separately. Background Data: The effects or LLLT on human cells are still poorly understood and unexplained. Several cell types were found non responsive to laser bio stimulation; in other cases, only a partial activation was observed. Methods: A laser device was used for cell irradiation with a continuous wave diode (λ = 808 nm), a pulsed wave diode (λ = 905 nm), and a combined wave diodes (λ = 808 nm + 905 nm), in the dose range or 1-60 J/cm2. Results: The effect of the combined low-level 808-905 nm diode laser irradiation were slightly superior to those achieved with either laser alone in HeLa cells. TK6 cellular proliferation was not round to be significantly affected by any or the energy levels and varying exposure doses investigated. Conclusions: Our results are a confirmation of previous observations carried out on human cells, where only the proliferation or slowly growing cell populations appeared to be stimulated by laser light.
Photomedicine and Laser Surgery Volume 22, Number 6, 2004 © Mary Ann Liebert, Ine.
Pp. 523-526 |
